Comparing transient oligonucleotide hybridization kinetics using DNA-PAINT and optoplasmonic single-molecule sensing on gold nanorods
Abstract
We report a comparison of two photonic techniques for single-molecule sensing: fluorescence nanoscopy and optoplasmonic sensing. As the test system, oligonucleotides with and without fluorescent labels are transiently hybridized to complementary docking strands attached to gold nanorods. Comparing the measured single-molecule kinetics helps to examine the influence of fluorescent labels as well as factors arising from different sensing geometries. Our results demonstrate that DNA dissociation is not significantly altered by the fluorescent label, while DNA association is affected by geometric factors in the two techniques. These findings open the door to exploiting plasmonic sensing and fluorescence nanoscopy in a complementary fashion, which will aid in building more powerful sensors and uncovering the intricate effects that influence the behavior of single molecules.
Keywords
Cite
@article{arxiv.2103.07520,
title = {Comparing transient oligonucleotide hybridization kinetics using DNA-PAINT and optoplasmonic single-molecule sensing on gold nanorods},
author = {Narima Eerqing and Sivaraman Subramanian and Jesús Rubio and Tobias Lutz and Hsin-Yu Wu and Janet Anders and Christian Soeller and Frank Vollmer},
journal= {arXiv preprint arXiv:2103.07520},
year = {2021}
}
Comments
8 pages, 3 figures, 1 table. Added an analysis for another DNA strand plus several control experiments