Modern microscopy techniques are aimed at imaging an individual molecule at atomic resolution. Here we show that low-energy electrons with kinetic energies of 50-250 eV offer a possibility of overcome the problem of radiation damage, and obtaining images of individual biomolecules. Two experimental schemes for obtaining images of individual molecules, holography and coherent diffraction imaging, are discussed and compared. Images of individual molecules obtained by both techniques, using low-energy electrons, are shown.
@article{arxiv.1305.1897,
title = {Coherent diffraction and holographic imaging of individual biomolecules using low-energy electrons},
author = {Tatiana Latychevskaia and Jean-Nicolas Longchamp and Conrad Escher and Hans-Werner Fink},
journal= {arXiv preprint arXiv:1305.1897},
year = {2013}
}
Comments
17 pages, 9 figures. See for the full text at Springer website http://link.springer.com/chapter/10.1007%2F978-94-007-6232-9_29# Present and Future Methods for Biomolecular Crystallography, Springer, 2013